ASTM E2149 PDF

ASTM E Antimicrobial Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact. ASTM E Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact Conditions. ASTM E Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents under Dynamic Contact Conditions. The test.

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ASTM E – Microbe Investigations (MIS)

This test ensures good contact between bacteria and the treated fibre by constant agitation of the test specimen in a bacterial suspension during the test period 1hour. The test determines the antimicrobial activity of a treated specimen by shaking samples of surface-bound materials in a concentrated bacterial suspension for a one hour contact time.

Historical Version s – view previous versions of standard. xstm

Link to Active This link will always route to the current Active version of the standard. No regulated reduction limits currently exist for general antimicrobial claims made using this method.

The susceptibility of the species to particular biocides could be altered depending on its life xstm cycle. It has been developed for routine quality control and screening tests and is used to evaluate substrate bound antimicrobials.

ASTM E2149 Antimicrobial Activity

In this method, treated test samples are placed in e214 laboratory flask asgm a dilute suspension of test organism, commonly Escherichia coli.

It is for this reason that the use of the term leaching throughout this document is limited to only the testing conditions described herein. Presence of residual antimicrobial activity indicates the presence of a leaching type of antimicrobial agent.

Following exposure, a sample of the test organism suspension is removed quantitatively assayed for survivors.

One-hour contact time in a buffer solution allows for metabolic stasis in the population. Summary of Test In this method, treated test samples are placed in a laboratory flask containing a dilute suspension of test organism, commonly Escherichia coli.

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This dynamic shake flash astj was developed for routine quality control and screening test. This method is generally used to substantiate antimicrobial properties of treated specimens for non-public health related claims.

This test method is only intended to determine efficacy as described in 4. This standard does not purport to address all of the safety concerns, if any, associated with its use. Surface antimicrobial activity is determined by comparing results from the test sample to controls run simultaneously. No other units of measurement are included in this standard.

Immobilized antimicrobial agents, such as surface bonded materials, are not free qstm diffuse into their environment under normal conditions of use.

The ASTM E Standard Test Method is used to evaluate the antimicrobial activity of substrate-bound, non-leaching, antimicrobial-treated samples under dynamic contact conditions.

Materials evaluated by this method may be comprised of fabric, paper, powder or other solid materials. Microbial concentrations in the treated and untreated samples are usually determined at 0, 1 or 24 hours contact time. To find out more about the cookies we use, see our Privacy Policy. Link to Active This link will always route to the current Active version of the standard. A single cookie will be used in your browser to remember your preference not to be tracked.

Work Item s – proposed revisions of this standard. Referenced Documents purchase separately The documents listed below are referenced within the subject standard but are not provided as part of the standard.

This test method standardizes both the growth conditions of the challenge species and substrate contact times to reduce the variability associated with growth phase of the microorganism. Asgm resulting plates are incubated, the number of survivors is enumerated and a percent reduction is determined for the test flask as compared to the untreated control suspension. Record and report presence of solution activity.

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Summary of Test Method: These difficulties include ensuring contact of inoculum to treated surface as in AATCCflexibility of retrieval at different contact times, use of inappropriately applied static conditions as in AATCCsensitivity, and e2194.

It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

This standard does not purport to address all of the safety concerns, if any, associated with its use.

It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Active view current version of standard. This test method is designed to evaluate the antimicrobial activity of non-leaching, antimicrobial-treated specimens under dynamic contact conditions.

All the samples are shaken in dynamic shake flasks in a wrist action shaker to ensure good contact between the bacteria and the treated fibre, fabric, or other substrate by constant agitation of the test specimen in a bacterial suspension during the test period. This dynamic shake flask test was e214 for routine quality control and screening tests in order to overcome difficulties in using classical antimicrobial test methods to evaluate substrate-bound antimicrobials.

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This website stores cookies on your computer. The number of viable organisms from the suspension is determined and the percent reduction is calculated by comparing retrievals from appropriate controls. Test Package Request Form.