CHARDAKOV METHOD PDF

2) In “Chardakov method for water potential measurement” experiment, you find that M solution has not been changed by water loss or absorption by the. Chardakov Technique. Plant Physiology. UNI. Incubating tissue in solution. Pre- incubation. Incubation. Post-incubation. Save for later measurement. Did solution change concentration? Solutes stay the same If water left or entered tissue –Water also entered or left solution –Solution becomes more dilute or.

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Record your data in Table 2. This technique for measuring water potential is similar in theory to the Chardakov method and shares the advantage chardwkov being simple to perform and doesn’t require expensive equipment. Cytology Osmosis and Water relations. Measuring Water Potential by the Gravimetric Technique.

Metjod water potential of the tissue is considered to be equal the osmotic potential of the incubating solution at which there is no change in tissue weight i.

Use the following equation to calculate the percent change in weight for each tissue by the following equation: Hypertonic Concentration with higher solute concentration and less water concentration Hypotonic lower solute concentration and more water concentration.

Cut them to the same length with a razor blade ca. Be sure not to include any fragments of the skin. Does it correspond to the value obtained by the Chardakov technique?

Divide by 10 6 to convert to MPa. What would happen to The size of the tissue? Water potential values determined by this method may be slightly more negative than those obtained by the Chardokov method. Metho Privacy Policy Feedback.

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Chardakov method

In this method, tissue samples are weighed before and after incubation in a series of solutions of known osmotic water potential. This occurs when the apoplast becomes infiltrated with water and solutes. Then remove the tissues, gently blot on paper towels and reweigh. Incubating tissue in solution Pre-incubationIncubation Post-incubation Save for later measurement. Determine the approximate sucrose concentration for which there is no net change in density after tissue incubation i.

Then, the percent change in weight of the tissue is plotted versus solution concentration or osmotic potential. Osmosis diffusion of water through a selectively permeable membrane Membrane X is permeable to water but not to protein Which side has the highest concentration.

The water potential measured by this technique should be the same as that obtained the Chardakov method. In both techniques, tissue samples are incubated in a series of solutions of known osmotic water potential. It is assumed that solute movement between tissue and solution is negligible. An alternate method to determine his point requires performing a regression analysis of the best fit line of your data. What is the water potential of potato tissue?

Does a gas differ from other fluids? OK Any substance that can change shape or flow easily. From the graph, determine the concentration of the sucrose solution in which there was no net weight gain i. Dispense 10 mL of water or sucrose 0. Record the temperature of the solutions Chardakiv 1 Using a Pasteur pipet, remove a small amount of water dyed with methylene blue to metyod the sucrose solution, dip a dry probe into methylene blue powder and then mix.

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Complete Tables 1 and 2. Use a cork borer to prepare at least 27 uniform tissue samples from the potato.

Change in weight of potato cores incubated in sucrose solutions. What is the water potential of potato tissue? Draw the best fit line for your data. The solution gains or looses water depending on the water potential of the tissue.

Boy, Oh Buoyancy Does it Float? Chardakov Method metjod Determining Water Potential. Two ways to move water Bulk flow Osmosis Both move water from high energy to low Differences —Source of energy difference. Periodically swirl the containers.

Investigating osmosis: measuring the water potential of a potato cell

Put two or preferably three potato cores in each solution water or sucrose. To use this website, you must agree to our Privacy Policyincluding cookie policy.

If the density merhod a solution does not change no net movement of water then this solution has the same water potential as the tissues that were incubated in it. Incubate the cores for 1. If you wish to download it, please recommend it to your friends in any social system.

One distinct advantage of this technique is that it provides a more accurate estimate of water potential. Auth with social network: